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アイテム
Genomic Control of Upregulation of GRP78 Expression for Promotion of Neurite Elongation and Attenuation of Cell Death via PKA-Mediated Signaling in PC12 Cells
http://hdl.handle.net/10112/11352
http://hdl.handle.net/10112/113525d848539-e83a-4224-9556-166bd6b7736b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KU-0020-20151123-00.pdf (489.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-07-06 | |||||
| タイトル | ||||||
| タイトル | Genomic Control of Upregulation of GRP78 Expression for Promotion of Neurite Elongation and Attenuation of Cell Death via PKA-Mediated Signaling in PC12 Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yamazoe, Ryosuke
× Yamazoe, Ryosuke× Nishihata, Yoshiki× Nakagawa, Kazuma× Aoyama, Hiroki× 下家, 浩二 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 1872 | |||||
| 姓名 | 山添, 亮輔 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 1873 | |||||
| 姓名 | 西畑, 慶紀 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 1874 | |||||
| 姓名 | 中川, 一馬 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 1875 | |||||
| 姓名 | 青山, 大輝 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 1876 | |||||
| 姓名 | 下家, 浩二 | |||||
| 概要 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Neurodegeneration occurs due to neuronal cell death and subsequently disrupts neuronal networks. In current medicine, methods for interruption of neuronal cell death and avoidance of disruption of neuronal networks have potential as therapy for neurodegenerative disorders. Development of this therapy requires analysis of the molecular mechanism of neurite extension that leads to network formation. Here, we show that forskolin (fsk), an activator of adenylate cyclase, increases the intracellular cAMP concentration and induces neurite outgrowth in PC12 cells. The effect of fsk on neurite outgrowth was diminished by H89, an inhibitor of protein kinase A (PKA), and by PD98059 and U0126, which are inhibitors of the mitogen-activated protein kinase (MAPK) signaling pathway. With fsk treatment in the presence of tunicamycin, an inducer of cell death, the activity of the glucose-regulated protein 78 (GRP78) promoters was upregulated. Interestingly, this effect was completely abolished by H89 and by PD98059 and U0126. This phenomenon was confirmed using a dominant-negative PKA-expressing PC12 cell line, in which the PKA-mediated signaling pathway was completely eliminated. These lines of evidence suggest that GRP78 promotes neuronal elongation that is regulated by fsk and mediated by PKA. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | This work was supported by SENRYAKU (2013-2017) and KAKENHI (25340104) grants-in-aid from MEXT (Ministry of Education, Culture, Sports, Science and Technology of Japan), and by a Kansai University Grant-in- Aid for Encouragement of Scientists (2014). | |||||
| 書誌情報 |
Clinical Pharmacology & Biopharmaceutics 巻 4, 号 4, p. 1-4, 発行日 2015-11-23 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2167065X | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.4172/2167-065X.1000150 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2015 Yamazoe R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | OMICS International | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | neurites | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | survival | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PC12 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | GRP78 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gene expression | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | genomics | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | epigenetics | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 関西大学 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Kansai University | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 平成26年度関西大学若手研究者育成経費 | |||||
