| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-11-17 |
| タイトル |
|
|
タイトル |
A novel type IIb L-asparaginase from Latilactobacillus sakei LK-145 : characterization and application |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
加藤, 志郎
田村, 和也
増田, 有紀
小西, 守周
山中, 一也
老川, 典夫
|
| 概要 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We succeeded in homogeneously expressing and purifying L-asparaginase from Latilactobacillus sakei LK-145 (Ls-Asn1) and its mutated enzymes C196S, C264S, C290S, C196S/C264S, C196S/C290S, C264S/C290S, and C196S/C264S/C290S Ls-Asn1. Enzymological studies using purified enzymes revealed that all cysteine residues of Ls-Asn1 were found to affect the catalytic activity of Ls-Asn1 to varying degrees. The mutation of Cys196 did not affect the specific activity, but the mutation of Cys264, even a single mutation, significantly decreased the specific activity. Furthermore, C264S/C290S- and C196S/C264S/C290S-Ls-Asn1 almost completely lost their activity, suggesting that C290 cooperates with C264 to influence the catalytic activity of Ls-Asn1. The detailed enzymatic properties of three single-mutated enzymes (C196S, C264S, and C290S-Ls-Asn1) were investigated for comparison with Ls-Asn1. We found that only C196S-Ls-Asn1 has almost the same enzymatic properties as that of Ls-Asn1 except for its increased stability for thermal, pH, and the metals NaCl, KCl, CaCl₂, and FeCl₂. We measured the growth inhibitory effect of Ls-Asn1 and C196S-Ls-Asn1 on Jurkat cells, a human T-cell acute lymphoblastic leukemia cell line, using L-asparaginase from Escherichia coli K-12 as a reference. Only C196S-Ls-Asn1 effectively and selectively inhibited the growth of Jurkat T-cell leukemia, which suggested that it exhibited antileukemic activity. Furthermore, based on alignment, phylogenetic tree analysis, and structural modeling, we also proposed that Ls-Asn1 is a so-called "Type IIb" novel type of asparaginase that is distinct from previously reported type I or type II asparaginases. Based on the above results, Ls-Asn1 is expected to be useful as a new leukemia therapeutic agent. |
|
言語 |
en |
| 書誌情報 |
en : Archives of Microbiology
巻 206,
号 6,
p. 1-14,
発行日 2024-05-18
|
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
0302-8933 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00548209 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s00203-024-03979-5 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00203-024-03979-5. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Lactic acid bacteria |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Latilactobacillus sakei |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
L-Asparaginase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Leukemia drugs |
| キーワード |
|
|
言語 |
ja |
|
主題Scheme |
Other |
|
主題 |
関西大学 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Kansai University |
KU-1100-20240518-01.pdf (5.1 MB)
