WEKO3
アイテム
Emulsion-Templated Preparation of Protein-Encapsulated Hydrophilic Polymeric Nanocapsules
http://hdl.handle.net/10112/0002003318
http://hdl.handle.net/10112/0002003318708a6692-ef1f-43a2-8654-a923722ba343
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KU-1100-20250507-00.pdf (1.5 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2026-05-07 | |||||||||||||||||||||
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| タイトル | Emulsion-Templated Preparation of Protein-Encapsulated Hydrophilic Polymeric Nanocapsules | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||
| 著者 |
河村, 暁文
× 河村, 暁文
WEKO
1885
× 清水, 美結
× 平林, 利香
× 宮田, 隆志
WEKO
54040
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| 概要 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Encapsulation of biomacromolecules, such as proteins, enzymes, and nucleic acids, is of greatinterest for applications in drug delivery systems and nanobioreactors, because it protects biomacromolecules from degradation by degrading enzymes. This paper describes the facile preparation of protein-encapsulated hydrophilic polymeric nanocapsules using an emulsion-templated approach. A water-soluble block copolymer emulsifier, poly(2- methacryloyloxyethyl phosphorylcholine)-b-poly[oligo(ethylene glycol)methacrylate] (PMPC-b-POEGMA), is synthesized via reversible addition fragmentation chain transfer polymerization. The PMPC-b-POEGMA emulsifier stabilizes the water-chloroform interface in water-in-oil emulsions. Nanocapsules are prepared by cross-linking the POEGMA blocks of the emulsifier located on the surface of the water droplets using divinyl sulfone (DVS). The resulting nanocapsules have a cross-linked POEGMA membrane and are stably dispersed in aqueous media without further modification. Peroxidase is encapsulated in nanocapsules by dissolving it in the water phase prior to emulsification and cross-linking. The cross-linking density of the capsule membrane can be tuned by varying the DVS to hydroxy group ratio, which affects the encapsulation efficiency. The nearly complete encapsulation of peroxidase is achieved when the DVS to hydroxy group ratio ranges from 0.1 to 1.0. This emulsion-templated strategy provides a facile route to protein-loaded hydrophilic nanocapsules which have potential applications in nanobioreactors, drug delivery systems, and diagnostic systems. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 書誌情報 |
en : Macromolecular Chemistry and Physics 巻 226, 号 14, p. 1-26, 発行日 2025-05-07 |
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||
| 収録物識別子 | 1022-1352 | |||||||||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||
| 収録物識別子 | 1521-3935 | |||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AA10984295 | |||||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1002/macp.202500022 | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 権利情報Resource | This is the peer reviewed version of the following article: A. Kawamura, M. Shimizu, R. Hirabayashi, T. Miyata, Emulsion-Templated Preparation of Protein-Encapsulated Hydrophilic Polymeric Nanocapsules. Macromol. Chem. Phys. 2025, 226, 70000, which has been published in final form at https://doi.org/10.1002/macp.202500022. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | WILEY-VCH | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 助成情報 | ||||||||||||||||||||||
| 助成機関名 | 日本学術振興会（JSPS） | |||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||||
| 研究課題番号 | 21K05202 | |||||||||||||||||||||
| 研究課題番号URI | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K05202/ | |||||||||||||||||||||
| 研究課題名 | Design of temperature-responsive nanocapsules controlling permeation of water-soluble compounds | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | water-dispersible polymeric nanocapsule | |||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | protein-encapsulated nanocapsule | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | W/O emulsion | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | in situ protein encapsulation | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | block copolymer emulsifier | |||||||||||||||||||||
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| 言語 | ja | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | 関西大学 | |||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Kansai University | |||||||||||||||||||||
