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IgA-enhancing effects of membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC15893
http://hdl.handle.net/10112/00019596
http://hdl.handle.net/10112/00019596ea395724-98c0-4ddc-8b79-2230b2ed6d93
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KU-1100-20181101-00.pdf (990.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-03-12 | |||||
| タイトル | ||||||
| タイトル | IgA-enhancing effects of membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC15893 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yamasaki-yashiki, Shino
× Yamasaki-yashiki, Shino× Miyoshi, Yuki× Nakayama, Tomoya× Kunisawa, Jun× Katakura, Yoshio |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 38680 | |||||
| 姓名 | 山崎（屋敷）, 思乃 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 38681 | |||||
| 姓名 | 中山, 友哉 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 38682 | |||||
| 姓名 | 片倉, 啓雄 | |||||
| 概要 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Immunoglobulin (Ig) A in the mucus of the intestinal tract plays an important role in preventing the invasion of pathogenic microorganisms and regulating the composition of the gut microbiota. Several strains of probiotic lactic acid bacteria (LAB) are known to promote intestinal IgA production. Bacteria are also known to naturally release spherical membrane vesicles (MVs) that are involved in various biological functions such as quorum sensing, pathogenesis, and host immunomodulation. However, the production of MVs by LAB and their effects on host immunity remain poorly understood. In this study, we investigated the MV production by Lactobacillus sakei subsp. sakei NBRC15893 isolated from kimoto, the traditional seed mash used for brewing sake. MVs were separated from the culture broth of L. sakei NBRC15893 through filtration and density gradient ultracentrifugation and were observed by transmission electron microscopy. The MVs showed a spherical morphology, with a diameter of 30–400 nm, and contained proteins and nucleic acids. In addition, both the LAB cells and purified MVs promoted IgA production by murine Peyer’s patch cells. This MV- and cell-induced IgA production was suppressed by neutralization of Toll-like receptor (TLR) 2, which recognizes cell wall components of gram-positive bacteria, using an anti-TLR2 antibody. Collectively, our results indicate that MVs released from L. sakei NBRC15893 enhance IgA production by activating host TLR2 signaling through its cell wall components. Thus, it is important to consider novel interactions between gut microbiota and hosts via MVs, and MVs derived from probiotic bacteria could have promising applications as safe adjuvants. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Japan Society for the Promotion of Science (JSPS) KAKENHI grant (Nos. 16K18302 and 18K04857 [to S.Y.Y]; 15H05790, 16H01373, 17H04134, and 26293111 [to J.K.]) | |||||
| 書誌情報 |
Bioscience of Microbiota, Food and Health 巻 38, 号 1, p. 23-29, 発行日 2018-11-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 21863342 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.12938/bmfh.18-015 | |||||
| 権利 | ||||||
| 権利情報 | (c) 2019 BMFH Press | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | BMFH Press | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | lactic acid bacteria | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | membrane vesicle | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gut immunity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | IgA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 関西大学 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Kansai University | |||||
| 出版者（他言語） | ||||||
| 値 | BMFH出版会 | |||||
