WEKO3
アイテム
Intramolecular Electron Transfer of Cytochrome c Modified with N,N,N′,N″,N″-Diethylenetriaminepentaacetatocobaltate(III)
http://hdl.handle.net/10112/6148
http://hdl.handle.net/10112/614813e5be43-5592-4bb4-b1e0-b08196690b84
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KU-1100-20030123-03.pdf (150.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-02-23 | |||||
| タイトル | ||||||
| タイトル | Intramolecular Electron Transfer of Cytochrome c Modified with N,N,N′,N″,N″-Diethylenetriaminepentaacetatocobaltate(III) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Tsukahara, Keiichi
× Tsukahara, Keiichi× Iida, Nao× Kaida, Yuka× Takashima, Hiroshi× Mizobe, Maya× Arakawa, Ryuichi |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 26446 | |||||
| 姓名 | 塚原, 敬一 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 26447 | |||||
| 姓名 | 飯田, 奈央 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 26448 | |||||
| 姓名 | 貝田, 有佳 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 26449 | |||||
| 姓名 | 高島, 弘 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 26450 | |||||
| 姓名 | 溝部, まや | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 26451 | |||||
| 姓名 | 荒川, 隆一 | |||||
| 概要 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Horse heart cytochrome c (cytc) modified with an N,N,N′,N″,N″-diethylenetriaminepentaacetatocobaltate(III) ion, [cytcIII{CoIII(dtpa)}], was prepared and characterized. The major component of singly modified [cytcIII{CoIII(dtpa)}] contains a [CoIII(dtpa)] ion at Lys13, of which the ε-amino group is linked with a carboxylate ion of dtpa. A one-electron reduced protein, [cytcII{CoIII(dtpa)}], was prepared by reduction with a methylviologen-radical cation, which was produced in situ by a photoreduction using a tris(2,2′-bipyridine)ruthenium(II) ion in the presence of disodium dihydrogen ethylenediaminetetraacetate at 25 °C, pH 7.5 (0.010 mol dm−3 tris(hydroxymethyl)aminomethane–HCl buffer), and an ionic strength of 0.50 mol dm−3 (NaCl). The reaction of [cytcII{CoIII(dtpa)}] to form [cytcIII{CoII(dtpa)}] was very slow, and was followed by conventional spectrophotometry. The reaction was of first order in the protein and the first-order rate constant ((3.1 ± 0.5) × 10−4 s−1) was independent of the concentration of the protein, indicating that the intramolecular electron-transfer (ET) process is predominant. The reaction mechanism is discussed on a through-bond or through-space pathway. The slow intramolecular ET rate might arise not only from the long distance, but also from the large reorganization energy for the Co(III)/Co(II) couple. | |||||
| 書誌情報 |
Bulletin of the Chemical Society of Japan 巻 76, 号 7, p. 1323-1328, 発行日 2003 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00092673 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00580132 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1246/bcsj.76.1323 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Chemical Society of Japan | |||||
| 出版者（他言語） | ||||||
| 値 | 日本化学会 | |||||
